Cat #: 11-322HC

Zymo Research D6012-FM Quick-DNA Fecal/Soil Microbe 96 Magbead Kit (Lysis Tubes), Zymo Research, 2 x 96 Preps/Unit

Zymo Research D6012-FM Quick-DNA Fecal/Soil Microbe 96 Magbead Kit (Lysis Tubes), Zymo Research, 2 x 96 Preps/Unit primary image
Zymo Research D6012-FM Quick-DNA Fecal/Soil Microbe 96 Magbead Kit (Lysis Tubes), Zymo Research, 2 x 96 Preps/Unit primary image
Zymo Research D6012-FM Quick-DNA Fecal/Soil Microbe 96 Magbead Kit (Lysis Tubes), Zymo Research, 2 x 96 Preps/Unit primary image

Cat #: 11-322HC

Zymo Research D6012-FM Quick-DNA Fecal/Soil Microbe 96 Magbead Kit (Lysis Tubes), Zymo Research, 2 x 96 Preps/Unit


Zymo Research

2 x 96 Preps/Unit

Brand: Zymo Research

  • Rapid, high-throughput method for the isolation of inhibitor-free, PCR-quality DNA from fecal samples, including those from humans, rats, cattle, etc
  • Ultra-high density BashingBeads are fracture resistant and chemically inert
  • Zymo MagBinding Bead technology effectively removes PCR inhibitors from the DNA product

Guest Price:
$1,170.40
List Price: $1,273.55
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Zymo Research

2 x 96 Preps/Unit

Brand: Zymo Research

  • Rapid, high-throughput method for the isolation of inhibitor-free, PCR-quality DNA from fecal samples, including those from humans, rats, cattle, etc
  • Ultra-high density BashingBeads are fracture resistant and chemically inert
  • Zymo MagBinding Bead technology effectively removes PCR inhibitors from the DNA product

The Quick-DNA Fecal/Soil Microbe 96 Magbead Kits are designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources, including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in as little as 90 minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Zymo MagBinding Bead technology, which features Zymo Research's Inhibitor Removal technology, is then used to isolate the DNA. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.

Brand Zymo Research Corporation
Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume >/= 37.5µl
Equipment Centrifuge fitted with a 96 well microplate carrier, 96 Well Magnetic Stand, Liquid handler or other robotic sample processor, 96 well plate heat block, 2ml 96 well plates and reagent carriers (user supplied).
Purity A260/A280nm >/=1.8.
Sample Source Feces or soil
Sample Storage DNA stored at </= -20°C.
Size Range Capable of recovering genomic DNA up to and above 40kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.
Type Total DNA
Yield </= 25µg total DNA

We have validated our kits with both high-speed homgenizers and low-speed disruptors. We highly recommend users to optimize their bead beating conditions for their own instruments. We recommend using a 2 ml-tube adapter to ensure that the bead beating is efficent (do not use adapters made of foam). For high-speed homogenizers, we recommend a total of 5 mins bead beating (1 min interval at 6.5 m/s with 5 mins rest, repeat 5 times). For low-speed cell disruptors, we recommend 30 mins at max speed.

Addition of beta-mercaptoethanol is recommended to enhance sample lysis, but can be substituted with dithiothreitol (DTT, final concentration of 10 mM). However, if bead beating is optimized and lysis is efficient, the addition of BME is not necessary and can be omitted.

No additional RNase A treatment is required when processing samples within kit capacity. The selective chemistry allows for binding of double stranded DNA to the column and for RNA to flow through.

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