DNase I cuts both double-stranded and single-stranded DNA, producing 3'-OH oligonucleotides. It is typically used for selectively degrading DNA in the presence of RNA. This DNase is suited for applications such as nick translation, production of random fragments, cleavage of genomic DNA for footprinting, removal of DNA template after in vitro transcription, and removal of DNA from RNA samples prior to applications such as RT-PCR. It is compatible with all of our RNA kits featuring in-column DNase digestion.

Unit Definition: One unit increases the absorbance of a high molecular weight DNA solution at a rate of 0.001 A260 units/min/ml of reaction mixture at 25°C (pH 5.0). (Kunitz, M. (1950) J Gen Physiol 33, 349 and 363)