Cat #: 11-707
Zymo Research E2017 DNA Degradase, Zymo Research, 2000U/Unit
Cat #: 11-707
Zymo Research E2017 DNA Degradase, Zymo Research, 2000U/Unit
Zymo Research
2000U/Unit
Brand: Zymo Research- Quick & simple procedure for completely degrading DNA into its individual nucleotide components for quantitative analysis
- 1 hour, single-enzyme digest vs. conventional 6 - 16-hour multi-step enzyme digestion protocols
$582.80
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Zymo Research
2000U/Unit
Brand: Zymo Research- Quick & simple procedure for completely degrading DNA into its individual nucleotide components for quantitative analysis
- 1 hour, single-enzyme digest vs. conventional 6 - 16-hour multi-step enzyme digestion protocols
DNA Degradase from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleotide components. DNA Degradase is ideal for whole-genome DNA methylation analysis by many downstream applications (i.e., HPLC, TLC, etc.). Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.
| Brand | Zymo Research Corporation |
|---|---|
| Assay Condition | DNA Degradase in 1X DNA Degradase Reaction Buffer. Incubate reaction mixtures at 37C for >/= 1 hour. |
| Concentration | 10U/µl |
| Enzyme Inactivation | 70C for 20 minutes |
| Storage | Store at -20C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at </= -70C. |
| Unit Definition | One unit (U) is the amount of enzyme required to degrade 1µg of lambda |
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Q1: Does this work with RNA/ ssDNA?
The preferred substrate for Degradase and Degradase Plus is double stranded DNA. There will be only minor degradation of single stranded DNA template and no degradation of RNA template.
Yes, you can scale the reaction up or down as necessary. For the best results, digest no more than 1µg of DNA with 5U (1µl) of enzyme in 25µl reaction volume. The reaction volume is just as important as the amount of DNA, and we recommend scaling up the volume of enzyme accordingly. For example, if the reaction volume is 100µl, use 4µl of DNA Degradase (Plus).
Yes, the protocol time is a suggestion for sufficient digestion. Additional incubation time can be added to completely ensure that all sample has been digested. There is no harm in letting the reaction proceed longer.
Yes, you can add excess enzyme to ensure full digestion.
The best way to confirm degradation is to run the sample on a gel. Nothing should be visible for the Degradase-treated samples. You do not need to purify the reaction.
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