Cat #: 13-015
Intact Genomics 1056-48 BL21(DE3) pLysS, Chemically Competent Cells, 12 x 200µl/Unit
Cat #: 13-015
Intact Genomics 1056-48 BL21(DE3) pLysS, Chemically Competent Cells, 12 x 200µl/Unit
Chemically Competent Cells
12 x 200µl/Unit
Brand: Intact Genomics- Suitable for transformation and routine protein expression
- Carry the chloramphenicol resistance gene
- Has T7 Expression Strain
- Deficient in both lon (1) and ompT proteases
- Resistant to phage T1 (fhuA2)
- B Strain
- Suitable for expression of toxic genes
$275.00
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Chemically Competent Cells
12 x 200µl/Unit
Brand: Intact Genomics- Suitable for transformation and routine protein expression
- Carry the chloramphenicol resistance gene
- Has T7 Expression Strain
- Deficient in both lon (1) and ompT proteases
- Resistant to phage T1 (fhuA2)
- B Strain
- Suitable for expression of toxic genes
Intact Genomics (ig®) chemically competent BL21(DE3)pLysS chemically competent cells are suitable for transformation and routine protein expression. The pLysS plasmid produces T7 lysozyme that reduces base-level expression of the gene of interest, subsequently allowing for tighter control of expression and is, therefore, suitable for the expression of toxic genes. BL21(DE3)pLysS chemically competent cells also carry the chloramphenicol resistance gene.
Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the high efficiency transformation protocol. Transformation efficiency should be greater than 3×10^7 CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.
| Competent cell type | Chemically competent |
|---|---|
| Derivative of | BL21(DE3)pLysS |
| Species | E. coli |
| Format | Tubes |
| Transformation efficiency | ?3 x 10^7 cfu/µg pUC19 DNA |
| Blue/white screening | Yes |
| Shipping condition | Dry ice |
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