Cat #: 13-060
Intact Genomics 1283-06 AGL1, Electrocompetent Agrobacterium, 6 x 50µl/Unit
Cat #: 13-060
Intact Genomics 1283-06 AGL1, Electrocompetent Agrobacterium, 6 x 50µl/Unit
Electrocompetent Agrobacterium
6 x 50µl/Unit
Brand: Intact Genomics- Allows for subsequent Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots
- Optimized for the highest transformation efficiencies
- Carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids
- Includes: AGL1 ElectroComp Agrobacterium, pCAMBIA1391z control DNA, and recovery medium
$198.00
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Electrocompetent Agrobacterium
6 x 50µl/Unit
Brand: Intact Genomics- Allows for subsequent Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots
- Optimized for the highest transformation efficiencies
- Carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids
- Includes: AGL1 ElectroComp Agrobacterium, pCAMBIA1391z control DNA, and recovery medium
Intact Genomics Agrobacterium tumefaciens AGL1 (AGL-1) ElectroCompetent cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. AGL1 electroporation cells with a binary vector allows for subsequent Agrobacterium-mediated transformation of Arabidopsis thaliana and other plants.
The AGL1 strain has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicilin resistance in its genome for selection. AGL1 contains the Ti plasmid pTiBO542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plants genome.
Competent cell type | Electrocompetent |
---|---|
Species | A. tumefaciens |
Strain | AGL1 |
Format | Tubes |
Transformation efficiency | ? 1 x 10^7 cfu/µg pCAMBIA1391z DNA |
Blue/white screening | No |
Shipping condition | Dry ice |
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