Intact Genomics Agrobacterium tumefaciens AGL1 (AGL-1) ElectroCompetent cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. AGL1 electroporation cells with a binary vector allows for subsequent Agrobacterium-mediated transformation of Arabidopsis thaliana and other plants.

The AGL1 strain has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicilin resistance in its genome for selection. AGL1 contains the Ti plasmid pTiBO542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome.