Intact Genomics (ig®) C58C1 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiency and are useful for various applications. The chromosomal background of C58C1 is C58. C58 is cured of the Ti plasmid pTiC58 resulting in C58C1. C58C1 Competent cells may be useful for transgenic operations that involve Arabidopsis and other plants. This Agrobacterium strain is streptomycin and rifampicin-resistant.

Transformation efficiency is tested by using the pCAMBIA1391z control DNA supplied with the kit and using the protocol in this manual. Transformation efficiency should be ?1 x 105 CFU/µg pCAMBIA1391z DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.