Cat #: 17-401
PCR Biosystems PB10.53-05 PCRBIO 1-Step Go RT-PCR Kit, 1 x 125ul RTase and 1 x 1.25ml 1-Step Go Mix, 50 Reactions/Unit
Cat #: 17-401
PCR Biosystems PB10.53-05 PCRBIO 1-Step Go RT-PCR Kit, 1 x 125ul RTase and 1 x 1.25ml 1-Step Go Mix, 50 Reactions/Unit
1 x 125ul RTase and 1 x 1.25ml 1-Step Go Mix
50 Reactions/Unit
Brand: PCR Biosystems- Thermostable reverse transcriptase RTase Go
- Broad temperature range 45°C to 55°C
- Advanced RNase inhibitor
- Antibody-mediated hot start technology for unrivalled detection of low copy number templates
- High PCR yields under standard and fast PCR conditions
- Efficient specific amplification from complex templates including GC and AT-rich sequences
- Use with any RNA template
Quantity
$236.15
1 x 125ul RTase and 1 x 1.25ml 1-Step Go Mix
50 Reactions/Unit
Brand: PCR Biosystems- Thermostable reverse transcriptase RTase Go
- Broad temperature range 45°C to 55°C
- Advanced RNase inhibitor
- Antibody-mediated hot start technology for unrivalled detection of low copy number templates
- High PCR yields under standard and fast PCR conditions
- Efficient specific amplification from complex templates including GC and AT-rich sequences
- Use with any RNA template
PCRBIO 1-Step Go RT-PCR Kit from Genesee Scientific is a convenient, easy-to-use kit for fast and efficient cDNA synthesis and PCR in a single tube.
The kit combines our modified thermostable reverse transcriptase (RTase Go) with an advanced RNase inhibitor to enhance cDNA synthesis speed and yield. The PCR step is powered by PCRBIO HS Taq DNA Polymerase giving robust RT-PCR performance with minimal or no optimisation required. Our antibody-mediated hot start technology prevents the formation of primer dimers and non-specific amplification giving improved reaction sensitivity and specificity compared to other methods. PCRBIO 1-Step Go RT-PCR Kit can be used with any RNA template including mRNA, total RNA and viral RNA sequences and gives reliable detection of even the most challenging targets.
| Packaging | 1 x 125ul RTase and 1 x 1.25ml 1-Step Go Mix |
|---|---|
| Quantity | 50 Reactions/Unit |
| Reverse Transcriptase | RTase Go |
| Compare To | Agilent AffinityScript One-Step RT-PCR Kit, QIAGEN OneStep RT-PCR Kit |
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Can I alter the activation time for the hot-start DNA polymerase?
We recommend using a minimum of 2 minutes for activating the polymerase. Longer times of up to 15 minutes can also be used with no detrimental effects to the enzyme.
1-step Both the cDNA synthesis and PCR reactions occur in the same mix. This option is suited for high throughput applications due to its speed and ease of set up. There is also a reduced risk of contamination. It is not ideal for low quality RNA samples or if the cDNA is required for archive or separate analysis. 2-step The cDNA synthesis and PCR reactions occur separately. This option is better suited if the cDNA product needs to be retained for analysis. It also allows for higher levels of reaction optimization. It permits control over the type and concentration of enzyme, RNA input and concentration of cDNA which, in turn, results in higher sensitivity compared to the 1-step format.
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