Cell freezing and storage at sub-zero temperatures is an important method for preserving cells for future studies. Cell freezing or cryopreservation ensures that you have a backup of cells in case of contamination and loss of cell supply. It is best to cryopreserve cells when they are at their maximum growth rate or almost confluent. Cell Freezing Medium B, when used with Cell Freezing Medium A, protects cells from damage after freezing and thawing. Cell Freezing Medium B (2X) is used with Cell Freezing Medium A (2X) in cell biology research for the cryopreservation of cells.

Recommendation: Suspend pelleted cells in cold Cell Freezing Medium A. Transfer to a freezing vial and add equal parts of cold Cell Freezing Medium B. Mix well and store by appropriate methods.