GelNest™ is a basement membrane product identical in composition and performance to Corning® Matrigel® Matrix. GelNest™ Matrix extracted from Engelbreth-Holm-Swarm (EHS) mouse sarcomas using genetically identical mouse lines and SOP's. EHS tumors contain plentiful ECM proteins such as laminin (60% ), collagen IV (30%), heparan sulfate proteoglycans, nidogen (8%), and several growth factors. Part number 211222 is the same best selling ECM formulation but without the phenol red indicator. Phenol red can exhibit estrogenic activity in some cell lines. Likewise removing the phenol red also makes this formulation ideal for colorimetric assays. Equivalent to Corning: 356237. Quality is guaranteed, each lot is produced and tested under stringent quality controls: Mouse colonies regularly tested for pathogens using mouse antibody production (MAP) protocol. Murine Engelbreth-Holm-Swarm (EHS) tumors used guaranteed free of LDEV. Per lot PCR testing for a wide variety of pathogens including LDEV. Tested and lot certified free of bacteria, fungi, and mycoplasma. Lowry method used to determine protein concentrations of >8mg/mL per lot. Limulus Amoebocyte Lysate assay used to quantify endotoxins, guaranteed <4EU/mL. Gel tested for 14 days at 37°C to guarantee stability. Lot tested using for biological activity using neurite growth and organoid formation assays.

211222 is the same best selling ECM formulation but without the phenol red indicator. Phenol red can exhibit estrogenic activity in some cell lines. Likewise removing the phenol red also makes this formulation ideal for colorimetric assays.